Endocrine Abstracts

Resistin, a novel signalling molecule isolated in mice, has been suggested to be the putative hormone thought to link obesity with type 2 diabetes. As central obesity is associated with a higher risk of diabetes, we compared resistin mRNA expression in abdominal fat depots to thigh and breast adipose tissue. Mononuclear cells are also known to expression resistin, therefore, CD45 expression (a marker of mononuclear blood cells), was also analysed. RNA was extracted from 32 hum...

Obesity-mediated diabetes is associated with insulin resistance/hyperinsulinaemia and chronic sub-clinical inflammation. Recent studies have highlighted fatty liver(steatosis) as a risk factor for diabetes. Fatty liver disease (FLD) progresses through fatty liver, necro-inflammation (non-alcoholic steatohepatitis, NASH) and fatwith fibrosis. In addition, hepatic steatosis per se can lead to hepatic insulin resistance/hyperin...

Chronic sub-clinical inflammation is associated with both type 2 diabetes and related vascular complications which is coupled with pro-inflammatory markers, such as IL-6, TNF-alpha and C-reactive protein (CRP). Resistin is the latest protein to be linked with inflammation and insulin resistance in obesity. Adipose tissue has been previously established as a source of circulating resistin, TNF-alpha and IL-6. As our previous studies demonstrated a correlation between serum resi...

Chronic sub-clinical inflammation is associated with increased risk of diabetes and cardiovascular disease and is particularly observed in patients that have undergone CABG. Resistin, a novel adipocyte derived protein, has been linked with central adiposity, insulin resistance and sub-clinical inflammation and may mediate both systemic and local paracrine pro-inflammatory responses in vivo. As such we investigated serum resistin from CABG patients and examined whether e...

Adiponectin is an adipocyte derived-hormone, associated with insulin sensitivity and a lower risk of atherosclerosis. As central rather than gluteo-femoral fat is known to increase the risk of type 2 diabetes (T2DM) and cardiovascular disease we investigated the mRNA and protein expression of adiponectin in human adipose tissue depots. RNA and protein was extracted using a cohort of 46 human adipose tissue samples (non-diabetic (ND) (age: (mean plus/minus SD) 44.33 plus/minus ...

(This poster is based on OC18.)...

Adiponectin (acrp30) is an adipocyte derived-hormone, associated with insulin sensitivity and lower risk of atherosclerosis. Plasma adiponectin concentrations are decreased in obese and type 2 diabetic subjects with insulin resistance. As central rather than gluteo-fermoral fat is known to increase the risk of type 2 diabetes and cardiovascular disease we investigated the expression of adiponectin in human adipose tissue depots. We examined expression of adiponectin mRNA level...

Mutations in the HSD11B2 gene explain the syndrome of apparent mineralocorticoid excess (AME), which is characterised by severe hypokalemic hypertension. The enzyme product of the HSD11B2 gene, 11-beta hydroxysteroid dehydrogenase type 2 (11beta HSD2), converts cortisol to its inactive form, cortisone. This reaction occurs primarily in the kidney, preventing the mineralocorticoid effects of cortisol, and in the placenta where it is believed to regulate fetal growth by protecti...

Mitochondrial dysfunction has been implicated in the pathogenesis of obesity and Type 2 Diabetes Mellitus (T2DM). Recent research has investigated the role of mitochondria in skeletal muscle and suggesting mitochondrial dysregulation may be linked to T2DM. To date few studies have focused on the role of human adipose tissue (AT) in energy homoeostasis. Therefore we investigated a number of mitochondrial genes including PPARγ co-activator1 (PGC1α, mitochondrial biogen...